利用球形芽孢杆菌C11的新型酶系统从淀粉生产环状四糖。

Production of cyclic tetrasaccharide from starch using a novel enzyme system from Bacillus globisporus C11.

作者信息

Aga Hajime, Higashiyama Takanobu, Watanabe Hikaru, Sonoda Tomohiko, Nishimoto Tomoyuki, Kubota Michio, Fukuda Shigeharu, Kurimoto Masashi, Tsujisaka Yoshio

机构信息

Amase Institute, Hayashibara Biochemical Laboratories, Inc., 7-7 Amase-minami-machi, Okayama 700-0834, Japan.

出版信息

J Biosci Bioeng. 2002;94(4):336-42. doi: 10.1263/jbb.94.336.

DOI:10.1263/jbb.94.336

PMID:16233313

Abstract

Production of cyclo[-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->] (CTS, cyclic tetrasaccharide) from starch was attempted using 1,6-alpha-glucosyltransferase (6GT) and 1,3-alpha-isomaltosyltransferase (IMT) from Bacillus globisporus C11. The optimal conditions for production from partially hydrolyzed starch were as follows: substrate concentration, 3%; pH 6-7; temperature, 30 degrees C; 6GT, 1 unit/g-dry solid (DS); IMT, 10 units/g-DS. The production of CTS was demonstrated and 544 g of CTS hydrate crystal powders were obtained from 3500 g of partially hydrolyzed starch. Two major by-products were also isolated from the reaction mixture and identified as the branched derivatives of CTSs, 4-O-alpha-D-glucopyranosyl-CTS and 3-O-alpha-isomaltosyl-CTS.

摘要

尝试使用球形芽孢杆菌C11的1,6-α-葡萄糖基转移酶（6GT）和1,3-α-异麦芽糖基转移酶（IMT）从淀粉生产环[->6)-α-D-吡喃葡萄糖-(1->3)-α-D-吡喃葡萄糖-(1->6)-α-D-吡喃葡萄糖-(1->3)-α-D-吡喃葡萄糖-(1->]（CTS，环状四糖）。从部分水解淀粉生产CTS的最佳条件如下：底物浓度3%；pH值6 - 7；温度30℃；6GT，1单位/克干固体（DS）；IMT，10单位/克DS。证实了CTS的生产，并且从3500克部分水解淀粉中获得了544克CTS水合物晶体粉末。还从反应混合物中分离出两种主要副产物，并鉴定为CTS的支链衍生物，4-O-α-D-吡喃葡萄糖基-CTS和3-O-α-异麦芽糖基-CTS。