用6-α-葡萄糖基转移酶和3-α-异麦芽糖基转移酶酶促合成一种由α-D-吡喃葡萄糖组成的新型环状五糖。

Enzymatic synthesis of a novel cyclic pentasaccharide consisting of alpha-D-glucopyranose with 6-alpha-glucosyltransferase and 3-alpha-isomaltosyltransferase.

作者信息

Watanabe Hikaru, Nishimoto Tomoyuki, Aga Hajime, Kubota Michio, Fukuda Shigeharu, Kurimoto Masashi, Tsujisaka Yoshio

机构信息

Amase Institute, Hayashibara Biochemical Laboratories, Inc., 7-7 Amase minami-machi, Okayama 700-0834, Japan.

出版信息

Carbohydr Res. 2005 Jul 4;340(9):1577-82. doi: 10.1016/j.carres.2005.04.017.

DOI:10.1016/j.carres.2005.04.017

PMID:15925352

Abstract

A novel cyclic pentasaccharide (CPS) and a branched cyclic pentasaccharide (6G-CPS) consisting of d-glucopyranose were synthesized with 6-alpha-glucosyltransferase (6GT) and 3-alpha-isomaltosyltransferase (IMT) from Bacillus globisporus N75. The structure of CPS was cyclo-[-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->]. The other, 6G-CPS, had the structure cyclo-[-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-alpha-D-Glcp-(1-->3)-[alpha-D-Glcp-(1-->6)]-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->]. The formation of CPS was presumed to occur after the following four successive reactions: a 6-glucosyltransfer reaction with 6GT, a 4-glucosyltransfer reaction with 6GT, a 3-isomaltosyltransfer reaction with IMT, and a cyclization reaction with IMT.

摘要

利用来自球形芽孢杆菌N75的6-α-葡萄糖基转移酶（6GT）和3-α-异麦芽糖基转移酶（IMT）合成了一种由D-吡喃葡萄糖组成的新型环状五糖（CPS）和一种支链环状五糖（6G-CPS）。CPS的结构为环-[→6)-α-D-葡萄糖-(1→3)-α-D-葡萄糖-(1→6)-α-D-葡萄糖-(1→3)-α-D-葡萄糖-(1→4)-α-D-葡萄糖-(1→]。另一种6G-CPS的结构为环-[→6)-α-D-葡萄糖-(1→3)-α-D-葡萄糖-(1→6)-α-D-葡萄糖-(1→3)-[α-D-葡萄糖-(1→6)]-α-D-葡萄糖-(1→4)-α-D-葡萄糖-(1→]。推测CPS的形成经过以下四个连续反应：与6GT的6-葡萄糖基转移反应、与6GT的4-葡萄糖基转移反应、与IMT的3-异麦芽糖基转移反应以及与IMT的环化反应。