Yun Zhanyou, Takagi Mutsumi, Yoshida Toshiomi
International Center for Biotechnology, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
J Biosci Bioeng. 2003;95(2):124-7.
The combined addition of reduced form of glutathione (GSH) and iron chelators for decrease of the intracellular level of reactive oxygen species (ROSs) and death of Chinese hamster ovary (CHO) cells was investigated. The addition of GSH to the serum-free cultures of CHO cells markedly lowered the intracellular ROS level, which effect was comparable to that of the addition of serum (0.4, 10%) and might be due to the reduction of ROS. The addition of iron chelators, deferoxamine and aurintricarboxylic acid, to the serum-free cultures also decreased the intracellular ROS level and increased the residual concentration of viable cells during the serum-free maintenance culture, which should be due to the inhibition of the Fenton and Harber-Weiss reactions stimulating the synthesis of ROS. The combined addition of GSH and the chelators decreased ROS level and cell death more effectively compared with their single addition.
研究了还原型谷胱甘肽（GSH）和铁螯合剂联合添加对降低中国仓鼠卵巢（CHO）细胞内活性氧（ROS）水平及细胞死亡的影响。向CHO细胞无血清培养物中添加GSH可显著降低细胞内ROS水平，其效果与添加血清（0.4%、10%）相当，这可能是由于ROS的减少。向无血清培养物中添加铁螯合剂去铁胺和金精三羧酸，也可降低细胞内ROS水平，并在无血清维持培养期间提高活细胞的残留浓度，这应该是由于抑制了刺激ROS合成的芬顿反应和哈伯-韦斯反应。与单独添加相比，GSH和螯合剂联合添加能更有效地降低ROS水平和细胞死亡。
