Overexpression of Aspergillus aculeatus cellobiohydrolase I in Aspergillus oryzae.
作者信息
Kanamasa Shin, Mochizuki Miho, Takada Goro, Kawaguchi Takashi, Sumitani Jun-Ichi, Araii Motoo
机构信息
Graduate School ofAgriculture and Biological Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan.
出版信息
J Biosci Bioeng. 2003;95(6):627-9.
PMID:16233469
Abstract
To express the cbhI gene, encoding Aspergillus aculeatus cellobiohydrolase I (CBHI), in Aspergillus oryzae, a plasmid was constructed. The strain that displayed the strongest CBHI activity among the transformants produced about 941 mg/l in liquid culture. It was confirmed by a PCR method that the plasmid was integrated at the niaD locus.
摘要
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