硫酸软骨素和透明质酸对软骨细胞三维培养中基因表达的影响。
Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes.
作者信息
Nishimoto Shohei, Takagi Mutsumi, Wakitani Shigeyuki, Nihira Takuya, Yoshida Toshiomi
机构信息
International Center for Biotechnology, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
出版信息
J Biosci Bioeng. 2005 Jul;100(1):123-6. doi: 10.1263/jbb.100.123.
The effect of glycosaminoglycan addition on a three-dimensional (3D) culture of porcine chondrocyte cells was investigated with a view to use in cartilage regenerative medicine. Chondroitin sulfate C increased the mRNA expression of type 2 collagen, while chondroitin sulfate A did not. Hyaluronic acid of high molecular weight markedly decreased the mRNA expression of both aggrecan and type 2 collagen, although hyaluronic acid of low molecular weight showed no apparent effect.
为了用于软骨再生医学，研究了添加糖胺聚糖对猪软骨细胞三维（3D）培养的影响。硫酸软骨素C增加了Ⅱ型胶原蛋白的mRNA表达，而硫酸软骨素A则没有。高分子量透明质酸显著降低了聚集蛋白聚糖和Ⅱ型胶原蛋白的mRNA表达，而低分子量透明质酸则没有明显影响。
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