Yang Jian-ming, Hu De-hui, Zhu Xin-hong, Gao Tian-ming
Department of Neurobiology, Southern Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2005 Oct;25(10):1225-7.
To study the localization of L-type calcium channel alpha1 subunits CaV1.2alpha1C and CaV1.3alpha1D in CA1 and CA3 regions of adult rat hippocampus.
Immunohistochemical staining was employed for specific labeling of alpha1 subunits CaV1.2alpha1C and CaV1.3alpha1D.
CaV1.2alpha1C subunit was mainly located in the apical and basal dendrites in the CA1 area and the CA3 area, neuronal soma, basal dendrites and distal apical dendrites were all positively stained. In contrast to CaV1.2alpha1C, CaV1.3alpha1D displayed no obvious difference in immunostaining between CA1 and CA3, and was distributed mainly in the neuronal soma and proximal dendrites. At cellular level, CaV1.2alpha1C distribution showed a distinct clustered pattern while a uniform distribution was observed for CaV1.3alpha1D subunit.
Different alpha1 subunits of L-type calcium channel have differential expression in adult hippocampal neurons.
研究L型钙通道α1亚基CaV1.2α1C和CaV1.3α1D在成年大鼠海马CA1和CA3区的定位。
采用免疫组织化学染色法对α1亚基CaV1.2α1C和CaV1.3α1D进行特异性标记。
CaV1.2α1C亚基主要位于CA1区和CA3区的顶端和基底树突，神经元胞体、基底树突和顶端远端树突均呈阳性染色。与CaV1.2α1C不同，CaV1.3α1D在CA1和CA3之间的免疫染色无明显差异，主要分布于神经元胞体和近端树突。在细胞水平上，CaV1.2α1C的分布呈明显的聚集模式，而CaV1.3α1D亚基则呈均匀分布。
L型钙通道的不同α1亚基在成年海马神经元中具有差异表达。
