髓母细胞瘤的细胞动力学

Cell kinetics of medulloblastomas.

作者信息

Ito S, Hoshino T, Prados M D, Edwards M S

机构信息

Department of Neurological Surgery, School of Medicine, University of California, San Francisco.

出版信息

Cancer. 1992 Aug 1;70(3):671-8. doi: 10.1002/1097-0142(19920801)70:3<671::aid-cncr2820700322>3.0.co;2-p.

DOI:10.1002/1097-0142(19920801)70:3<671::aid-cncr2820700322>3.0.co;2-p

PMID:1623482

Abstract

BACKGROUND

Cell kinetics and clinical behavior were correlated for 22 primary and 10 recurrent or metastatic medulloblastomas from 30 patients.

METHODS

Twenty-six patients received bromodeoxyuridine (BUdR) intravenously during craniotomy; excised specimens were stained by the immunoperoxidase method. The BUdR labeling index (LI) was calculated. Four patients received a pulse of tritiated thymidine; LI was calculated autoradiographically.

RESULTS

The mean LI for 32 tumors (11.7 +/- 1.3% standard error) indicated high proliferative potential. LI appeared higher in tumors from younger patients and those in the cerebellar hemisphere as compared with those in the cerebellar vermis. LI did not correlate with survival time. In six BUdR-labeled tumors labeled with iododeoxyuridine, the S-phase duration was 6.1-11.3 hours (mean, 8.0 +/- 0.8 hours); the potential doubling time was 25-82 hours. The actual doubling time, estimated in three cases from serial imaging, was 20-24 days. A cell loss factor of approximately 0.90 was assumed.

CONCLUSION

A high LI may reflect rapid medulloblastoma growth, but survival seemed more dependent on sensitivity to radiation therapy.

摘要

背景

对30例患者的22例原发性和10例复发性或转移性髓母细胞瘤的细胞动力学与临床行为进行了相关性研究。

方法

26例患者在开颅手术期间静脉注射溴脱氧尿苷（BUdR）；切除的标本采用免疫过氧化物酶法染色。计算BUdR标记指数（LI）。4例患者接受了氚标记胸腺嘧啶脉冲注射；通过放射自显影法计算LI。

结果

32个肿瘤的平均LI（标准误差为11.7±1.3%）表明其具有较高的增殖潜能。与小脑蚓部的肿瘤相比，年轻患者以及小脑半球肿瘤的LI似乎更高。LI与生存时间无关。在6个用碘脱氧尿苷标记的BUdR标记肿瘤中，S期持续时间为6.1 - 11.3小时（平均为8.0±0.8小时）；潜在倍增时间为25 - 82小时。根据连续成像在3例中估计的实际倍增时间为20 - 24天。假定细胞丢失因子约为0.90。