Oliviero Giorgia, Amato Jussara, Borbone Nicola, Galeone Aldo, Varra Michela, Piccialli Gennaro, Mayol Luciano
Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli Federico II, Via D. Montesano 49, I-80131 Napoli, Italy.
Biopolymers. 2006 Feb 15;81(3):194-201. doi: 10.1002/bip.20399.
The solid phase syntheses of the bunch oligonucleotides and based on the sequences of the natural oligodeoxynucleotides (ODNs) d(TG2TG2C) and d(CG2TG2T), respectively, attached to a non-nucleotidic tetrabranched linker, are reported. Bunch-ODNs and were shown to form more stable monomolecular parallel G-quadruplexes and when compared with their tetramolecular counterparts [d(TG2TG2C)]4 and [d(CG2TG2T)]4, respectively. The structure and stability of all the synthesized complexes have been investigated by circular dichroism (CD), CD thermal denaturation experiments, and 1H-NMR (nuclear magnetic resonance) experiments at variable temperatures. Particularly, the spectroscopic data confirmed that 1 adopts a T-tetrad containing parallel-stranded quadruplex structure as in the tetramolecular complex.
报道了分别基于天然寡聚脱氧核苷酸（ODN）d(TG2TG2C)和d(CG2TG2T)的序列，连接到非核苷酸四分支连接体上的束状寡核苷酸的固相合成。结果表明，与它们的四聚体对应物[d(TG2TG2C)]4和[d(CG2TG2T)]4相比，束状ODN分别形成更稳定的单分子平行G-四链体。通过圆二色性（CD）、CD热变性实验和可变温度下的1H-核磁共振（NMR）实验研究了所有合成复合物的结构和稳定性。特别地，光谱数据证实1采用了与四聚体复合物中相同的含T-四联体的平行链四链体结构。
