[老年口腔黏膜上皮异常增生中p53和Ki-67基因的表达及临床意义]

[Expression of p53 and Ki-67 genes in epithelial dysplasia from old oral mucosa and clinical significance].

作者信息

Zhao Jin, Guo Bin, Ma San-Cheng, Zhou Xue-Dong

机构信息

Department of Oral Medicine, West China School of Stomatology, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Sep;36(5):689-91.

PMID:16235539

Abstract

OBJECTIVE

To elucidate the relationship between the malignant transformation of oral epithelial dysplasia and the expression of p53 and Ki-67 proteins.

METHODS

The expression of p53 and Ki-67 proteins from 60 cases of oral epithelial dysplasia (28 cases of mild epithelial dysplasia, 32 cases of severe epithelial dysplasia) and 30 normal oral mucosa tissue was determined by immunihistochemistry, and a 5-year follow-up study was made.

RESULTS

In normal epithelium, Ki-67 expression was only observed in a small number of cells, and no expression of p53 was detected. In the cases of mild epithelial dysplasia, low levels of Ki-67 and p53 expression were detected. In the cases of severe epithelial dysplasia, the expression of p53 and Ki-67 protein was significantly higher than that in the normal oral mucosa and in the mild epithelial cases (P < 0.05). There was a correlation between the malignant transformation and the mutation of p53, Ki-67 in the cases of oral epithelial dysplasia.

CONCLUSION

Carcinogenesis of oral mucosa is a continuous and stepwise course; p53 and Ki-67 genes may play an important role in this course.

摘要

目的

阐明口腔上皮发育异常的恶性转化与p53和Ki-67蛋白表达之间的关系。

方法

采用免疫组织化学法检测60例口腔上皮发育异常（轻度上皮发育异常28例，重度上皮发育异常32例）及30例正常口腔黏膜组织中p53和Ki-67蛋白的表达，并进行5年随访研究。

结果

在正常上皮中，仅在少数细胞中观察到Ki-67表达，未检测到p53表达。在轻度上皮发育异常病例中，检测到低水平的Ki-67和p53表达。在重度上皮发育异常病例中，p53和Ki-67蛋白的表达明显高于正常口腔黏膜和轻度上皮病例（P<0.05）。口腔上皮发育异常病例的恶性转化与p53、Ki-67的突变之间存在相关性。