口腔上皮发育异常中Ki-67、p63、CK19和p53表达的免疫组织学评估
Immunohistological evaluation of Ki-67, p63, CK19 and p53 expression in oral epithelial dysplasias.
作者信息
Takeda T, Sugihara K, Hirayama Y, Hirano M, Tanuma J-I, Semba I
机构信息
Department of Maxillofacial Diagnostic and Surgical Sciences, Field of Oral and Maxillofacial Rehabilitation, Kagoshima University Graduate School of Medical and Dental Sciences, Sakuragaoka 8-35-1, Kagoshima 890-8544, Japan.
出版信息
J Oral Pathol Med. 2006 Jul;35(6):369-75. doi: 10.1111/j.1600-0714.2006.00444.x.
BACKGROUND
Oral squamous cell carcinoma develops through a multistep of genetic mutations, and the process can be morphologically recognized as oral epithelial dysplasia. To evaluate the hypothesis that distributional alterations of proliferating and stem cells may be a useful index to estimate the grading and development of epithelial dysplasia, we examined the distribution patterns according to stratified cell layers.
METHODS
Sixty-two oral dysplasia cases according to the histological grades were immunohistologically examined and the nuclear expression of Ki-67 and p63 antigens was counted according to epithelial layers as labeling index.
RESULTS
The Ki-67 labeling index in the basal and suprabasal layers and that of p63 in the basal layer showed a significant difference between low- and high-grade groups of epithelial dysplasia.
CONCLUSION
The architectural alteration of proliferating cell and stem cell distribution in the layers of epithelial dysplasias may provide useful information to evaluate the grading of oral epithelial dysplasias.
背景
口腔鳞状细胞癌通过多步骤基因突变发展而来，该过程在形态学上可被识别为口腔上皮发育异常。为评估增殖细胞和干细胞的分布改变可能是估计上皮发育异常分级和发展的有用指标这一假说，我们根据分层细胞层检查了分布模式。
方法
根据组织学分级对62例口腔发育异常病例进行免疫组织化学检查，并根据上皮层将Ki-67和p63抗原的核表达计数为标记指数。
结果
上皮发育异常的低级别和高级别组之间，基底层和基底上层的Ki-67标记指数以及基底层的p63标记指数存在显著差异。
结论
上皮发育异常各层中增殖细胞和干细胞分布的结构改变可能为评估口腔上皮发育异常的分级提供有用信息。
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