Thiagarajan S, Jeya M, Gunasekaran P
Department of Microbial Technology, Center for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021, India.
Indian J Exp Biol. 2005 Oct;43(10):887-91.
Optimization of media for the maximum production of xylanase by Aspergillus fumigatus MKUI was carried out using De Meo's fractional factorial design with seven components such as NaNO3, K2HPO4, MgSO4, FeSO4. KCl, peptone and yeast extract. A. fumigatus produced a maximum of 700 U/gds of enzyme after 48 hr of incubation (before optimization). After two steps of optimization, the medium designed favoured a 2.8 fold (1950 U/gds) increase in xylanase production by A. fumigatus. Optimized medium for Aspergillus fumigatus contained (g/l) NaNO3, 15; K2HPO4, 15; MgSO4, 5; FeSO4, 0.009; KCI, 0.5; peptone, 20; and yeast extract, 10.
使用De Meo部分因子设计，以硝酸钠、磷酸氢二钾、硫酸镁、硫酸亚铁、氯化钾、蛋白胨和酵母提取物等七种成分，对烟曲霉MKUI产木聚糖酶的培养基进行了优化。烟曲霉在培养48小时后（优化前）产生的酶量最高为700 U/gds。经过两步优化后，设计的培养基使烟曲霉产生的木聚糖酶增加了2.8倍（1950 U/gds）。烟曲霉的优化培养基含有（g/l）：硝酸钠15、磷酸氢二钾15、硫酸镁5、硫酸亚铁0.009、氯化钾0.5、蛋白胨20和酵母提取物10。
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