Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, Siddarthanagar, Mysore, India.
Bioresour Technol. 2010 Oct;101(19):7563-9. doi: 10.1016/j.biortech.2010.04.033. Epub 2010 May 15.
The effects of solid substrates, initial moisture content, moistening medium, temperature and incubation time on xylanase production by Aspergillus niger DFR-5 was studied and the highest activity (2596 IU/g dry substrate (gds)) was achieved in medium that contained wheat bran (WB) and soybean cake (SBC) at a ratio of 70:30, was moistened to 70% with MSS-2 mineral salt solution, and incubated for 6 days at 40 degrees C. Water at 37 degrees C was suitable for efficient recovery of enzyme from moldy WB-SBC medium. The extraction parameters for xylanase were optimized with respect to minimum volume of extractant using a central composite rotatable design (CCRD). The maximum recovery of xylanase (4465+/-52 IU/gds) with 92.5% desirability was obtained employing water (10 ml/gds) as extractant at 200 rpm for 60 min. The result shows that an overall 5.4-fold increase in xylanase production was obtained in concentrated form by optimizing medium components and extraction conditions.
研究了固体基质、初始含水量、润湿介质、温度和培养时间对黑曲霉 DFR-5 木聚糖酶生产的影响，在含有麦麸（WB）和豆饼（SBC）比例为 70:30 的培养基中，用 MSS-2 无机盐溶液润湿至 70%，在 40°C 下培养 6 天，达到最高酶活（2596IU/g 干基质（gds））。37°C 的水适合从发霉的 WB-SBC 培养基中高效回收酶。采用中心复合旋转设计（CCRD），对提取剂的最小体积进行了木聚糖酶提取参数的优化。采用水（10ml/gds）作为提取剂，在 200rpm 下提取 60min，获得了最大木聚糖酶回收率（4465+/-52IU/gds），达到 92.5%的理想水平。结果表明，通过优化培养基成分和提取条件，木聚糖酶的产量在浓缩形式下总体提高了 5.4 倍。
