Mostafa A M, Fields P G, Holliday N J
Department of Entomology, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2.
J Invertebr Pathol. 2005 Oct;90(2):79-84. doi: 10.1016/j.jip.2005.08.007. Epub 2005 Oct 19.
While maintained under all combinations of three temperatures and two RH, fifth instar larvae of the Mediterranean flour moth, Ephestia kuehniella were fed wheat treated with spores and crystals of Bacillus thuringiensis var. kurstaki. Larvae that had fed on wheat with the bacterial preparation contained higher concentrations of nodules in their haemocoel than did larvae fed on wheat without bacteria. Nodule concentrations in larvae fed untreated wheat were unaffected by temperature or relative humidity. Larvae fed treated wheat had higher nodule concentrations at 32 degrees C than at 15 and 23 degrees C, and higher nodule concentrations at a relative humidity of 85% than at 43%. The percentage of larvae that pupated was lower when larvae were fed the bacterial preparation, and was significantly higher at 23 degrees C than at 15 and 32 degrees C, regardless of whether larvae were fed bacteria or not. Less time was required for larvae to develop to pupation at higher temperatures and at higher humidity. Mean time to pupation was lower for bacteria-fed larvae than for those fed untreated wheat, and this appeared to be a result of truncation of the distribution of times to pupation because only rapidly developing larvae survived to pupation.
在地中海粉螟（Ephestia kuehniella）的五龄幼虫处于三种温度和两种相对湿度的所有组合条件下时，用苏云金芽孢杆菌库尔斯塔克变种（Bacillus thuringiensis var. kurstaki）的孢子和晶体处理过的小麦喂养它们。取食含有该细菌制剂小麦的幼虫，其血腔中的结节浓度高于取食不含细菌小麦的幼虫。取食未处理小麦的幼虫体内的结节浓度不受温度或相对湿度的影响。取食处理过小麦的幼虫在32℃时的结节浓度高于15℃和23℃时的浓度，在相对湿度为85%时的结节浓度高于43%时的浓度。当幼虫取食该细菌制剂时，化蛹幼虫的比例较低，并且无论幼虫是否取食细菌，在23℃时化蛹幼虫的比例显著高于15℃和32℃时的比例。幼虫在较高温度和较高湿度下发育至化蛹所需的时间更短。取食细菌的幼虫化蛹的平均时间比取食未处理小麦的幼虫短，这似乎是化蛹时间分布被截断的结果，因为只有发育迅速的幼虫才能存活至化蛹。
