Zhang Ze Sheng, James Anthony E, Huang Yu, Ho Walter K K, Sahota Daljit S, Chen Zhen-Yu
College of Food Science and Bioengineering, Tianjin University of Science and Technology, Tianjin, China.
Int J Food Sci Nutr. 2005 Aug;56(5):359-66. doi: 10.1080/09637480500170564.
Determination of fat percentage of aortic intimal area stained by Sudan III is useful as an index of atherosclerosis in the rabbit animal model. However, the determination of sudanophilic area of the thoracic aorta is two-dimensional and does not measure the third dimension of depth. The objective of the present study was to quantify and characterize aortic lipids using the gas-liquid chromatographic (GLC) technique and to determine whether elevated measurements of total cholesterol and cholesteryl esters was correlated with increased measurements of sudanophilic area staining of the thoracic aorta in rabbits given either a normal chow or a 1% cholesterol diet. The GLC results showed that there was a mean accumulation of 10.9 mg of cholesterol per gram of aortic tissue in the rabbits given a cholesterol diet (mean sudanophilic area of 23.8%). In contrast, rabbits on a normal chow diet had only a deposition of 0.58 mg of cholesterol per gram of the aortic tissue diet (mean sudanophilic area of 1.4%). The present study suggests that quantification of the aortic lipids can be performed by using GLC techniques and that it could be used as an alternative to the measurement of sudanophilic area when assessing the severity of atherosclerosis.
在兔动物模型中，测定经苏丹III染色的主动脉内膜区域的脂肪百分比，可作为动脉粥样硬化的一个指标。然而，胸主动脉嗜苏丹区域的测定是二维的，并未测量深度这一第三维度。本研究的目的是使用气液色谱（GLC）技术对主动脉脂质进行定量和表征，并确定在给予正常饲料或1%胆固醇饲料的兔子中，总胆固醇和胆固醇酯的测量值升高是否与胸主动脉嗜苏丹区域染色测量值的增加相关。GLC结果显示，给予胆固醇饲料的兔子每克主动脉组织平均积累10.9毫克胆固醇（平均嗜苏丹区域为23.8%）。相比之下，正常饲料喂养的兔子每克主动脉组织仅沉积0.58毫克胆固醇（平均嗜苏丹区域为1.4%）。本研究表明，可使用GLC技术对主动脉脂质进行定量，并且在评估动脉粥样硬化严重程度时，它可作为嗜苏丹区域测量的替代方法。
