短期分级力竭运动对血清脂质氧化易感性的影响。

Dayan A, Rotstein A, Pinchuk I, Vodovicz A, Lencovski Z, Lichtenberg D, Inbar O

Department of Physiology and Pharmacology, Tel-Aviv University, Sackler School of Medicine, Tel-Aviv, Israel.

Int J Sports Med. 2005 Nov;26(9):732-8. doi: 10.1055/s-2005-837447.

The objective of our study was to evaluate the effect of short-term intensive exercise on the susceptibility of serum lipids to ex-vivo peroxidation. We assessed the association between aerobic capacity, serum composition, and serum lipid oxidizability as well as the association between aerobic capacity and the effect of short-term maximal exercise on the kinetics of ex-vivo copper-induced peroxidation of serum lipids. The study involved 30 healthy male volunteers (age 22-39 years, BMI 19.4-29.8). Following 12-hr fasting, blood was withdrawn for determination of blood lipids, LDL, HDL, and TG, and Vitamin E, and for oxidizability assay of the serum lipids. Subsequently, each volunteer underwent an incremental all-out cardiopulmonary exercise stress test (CPET), performed on a motor-driven treadmill (Quinton Q65, USA). The test protocol was a modified Balke protocol. The results of this test were expressed in terms of mass-dependent maximal oxygen uptake (VO2max, ml.kg(-1).min (-1)) and of ventilatory anaerobic threshold (VAT, ml.kg(-1).min(-1)). Immediately after exercise, blood was re-drawn for the determination of serum Vitamin E and for ex-vivo oxidizibility assay, expressed in terms of maximal absorption of oxidation products (OD(max), absorbance units), maximal rate of their production (V(max), OD min(-1)) and the time at which the rate was maximal (t(max), min). Maximal graded exercise had no significant effect on the susceptibility of serum lipids to peroxidation as measured by OD(max) (p = 0.38 at 245 nm, and 0.27 at 268 nm),V(max) (p = 0.34 at 245 nm, and 0.49 at 268 nm) and t(max) (p = 0.17 at 245 nm, and 0.07 at 268 nm). Also no effect was found on the concentration of serum Vitamin E (p = 0.39). Aerobic capacity was not associated either with the susceptibility of serum lipids to ex-vivo peroxidation or with serum Vitamin E concentration. The present findings indicate that a short graded maximal exercise, lasting 8-12 min, is not sufficient to increase the susceptibility of the serum lipids to peroxidation. Thus it may be assumed that the antioxidant capacity of most healthy subjects provides proper protection from a short exhaustive exercise challenge. Also, aerobic capacity in the range represented by our subjects does not seem to influence the susceptibility of serum lipids to peroxidation.

我们研究的目的是评估短期强化运动对血清脂质体外过氧化敏感性的影响。我们评估了有氧能力、血清成分与血清脂质氧化能力之间的关联，以及有氧能力与短期最大运动对血清脂质体外铜诱导过氧化动力学影响之间的关联。该研究纳入了30名健康男性志愿者（年龄22 - 39岁，体重指数19.4 - 29.8）。在禁食12小时后，采集血液用于测定血脂、低密度脂蛋白、高密度脂蛋白、甘油三酯和维生素E，以及进行血清脂质的氧化能力测定。随后，每位志愿者在电动跑步机（美国昆顿Q65）上进行递增式全力心肺运动应激试验（CPET）。试验方案为改良的巴尔克方案。该试验结果以质量依赖的最大摄氧量（VO2max，毫升·千克⁻¹·分钟⁻¹）和通气无氧阈（VAT，毫升·千克⁻¹·分钟⁻¹）表示。运动结束后立即再次采集血液，用于测定血清维生素E和进行体外氧化能力测定，以氧化产物的最大吸光度（OD(max)，吸光度单位）、其产生的最大速率（V(max)，OD·分钟⁻¹）以及速率最大时的时间（t(max)，分钟）表示。最大分级运动对血清脂质过氧化敏感性无显著影响，以OD(max)衡量（245纳米处p = 0.38，268纳米处p = 0.27），V(max)（245纳米处p = 0.34，268纳米处p = 0.49）和t(max)（245纳米处p = 0.17，268纳米处p = 0.07）。对血清维生素E浓度也未发现影响（p = 0.39）。有氧能力与血清脂质体外过氧化敏感性或血清维生素E浓度均无关联。目前的研究结果表明，持续8 - 12分钟的短期分级最大运动不足以增加血清脂质的过氧化敏感性。因此可以推测，大多数健康受试者的抗氧化能力能为其提供适当保护，使其免受短期力竭运动的挑战。此外，我们研究对象所代表的有氧能力范围似乎并不影响血清脂质的过氧化敏感性。