新型嗜酸耐热芽孢杆菌属分离培养基的研发
Development of novel Alicyclobacillus spp. isolation medium.
作者信息
Chang S, Kang D-H
机构信息
Department of Food Science and Human Nutrition, Washington State University, Pullman, WA 99164, USA.
出版信息
J Appl Microbiol. 2005;99(5):1051-60. doi: 10.1111/j.1365-2672.2005.02716.x.
AIM
To develop a new isolation medium with higher recovery rates of Alicyclobacillus spp.
METHODS AND RESULTS
SK agar was developed with optimized incubation temperature, pH, acidulant, Tween 80 concentration and divalent cation addition. Results indicate that detection of Alicyclobacillus spp. by SK agar was significantly higher (P > 0.05) than those obtained by K agar, orange serum agar, and potato dextrose agar.
CONCLUSIONS
Current media used for Alicyclobacillus spp. isolation still resulted in high numbers of false negative products. The sensitivity of SK agar to Alicyclobacillus spp. allows detection of low numbers of Alicyclobacillus spp. and also provides a more higher isolation results compared with currently used media.
SIGNIFICANCE AND IMPACT OF THE STUDY
SK agar will be useful to the fruit juice industry to obtain more accurate numbers of contaminant Alicyclobacillus spp. With this media, false negative samples can be reduced, and the likelihood of exported products being rejected can be greatly reduced.
目的
开发一种能提高 Alicyclobacillus 属细菌回收率的新型分离培养基。
方法与结果
通过优化培养温度、pH 值、酸化剂、吐温 80 浓度和二价阳离子添加量来研制 SK 琼脂。结果表明，用 SK 琼脂检测 Alicyclobacillus 属细菌的结果显著高于（P > 0.05）用 K 琼脂、橙色血清琼脂和马铃薯葡萄糖琼脂所获得的结果。
结论
目前用于分离 Alicyclobacillus 属细菌的培养基仍会导致大量假阴性结果。SK 琼脂对 Alicyclobacillus 属细菌的敏感性能够检测到少量的 Alicyclobacillus 属细菌，并且与目前使用的培养基相比，能提供更高的分离结果。
研究的意义和影响
SK 琼脂将有助于果汁行业更准确地获取污染的 Alicyclobacillus 属细菌数量。使用这种培养基，可以减少假阴性样本，大大降低出口产品被拒收的可能性。
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