评估用于复苏嗜热栖热放线菌属物种的不同生长培养基。
Evaluation of different growth media for the recovery of the species of Alicyclobacillus.
作者信息
Witthuhn R C, Duvenage W, Gouws P A
机构信息
Department of Food Science, Stellenbosch University, Stellenbosch, Matieland, 7602, South Africa.
出版信息
Lett Appl Microbiol. 2007 Aug;45(2):224-9. doi: 10.1111/j.1472-765X.2007.02182.x.
AIMS
Five different isolation media, namely potato dextrose agar (PDA), orange serum agar (OSA), K agar, yeast-starch-glucose agar and Bacillus acidocaldarius medium were evaluated for the recovery of Alicyclobacillus spp. from inoculated diluted and undiluted fruit-juice concentrates.
METHODS AND RESULTS
Plates of PDA (pH 3.7), spread with vegetative cells (3.9 x 10(6) CFU ml(-1)) of Alicyclobacillus acidoterrestris from single-strength pear juice, recovered 2.9 x 10(6 )CFU ml(-1) after 5 days at 50 degrees C (74% recovery). The recovery of endospores from single-strength pear juice, after a heat treatment at 80 degrees C for 10 min, was higher on spread plates of OSA (pH 5.5) at 50 degrees C for 5 days (97% recovery).
CONCLUSIONS
PDA (pH 3.7) and OSA (pH 5.5) at 50 degrees C for 3-5 days recovered the highest numbers of vegative cells and endospores of Alicyclobacillus spp. from sterilized fruit juices and concentrates.
SIGNIFICANCE AND IMPACT OF THE STUDY
The most appropriate synthetic media for the recovery of Alicyclobacillus species from inoculated fruit juices and concentrates are shown.
目的
评估五种不同的分离培养基，即马铃薯葡萄糖琼脂（PDA）、橙汁血清琼脂（OSA）、K琼脂、酵母 - 淀粉 - 葡萄糖琼脂和嗜酸热芽孢杆菌培养基，用于从接种的稀释和未稀释果汁浓缩物中回收 Alicyclobacillus 属细菌。
方法与结果
用来自单倍浓缩梨汁的嗜酸热 Alicyclobacillus 营养细胞（3.9×10⁶ CFU ml⁻¹）涂布在pH 3.7的PDA平板上，在50℃培养5天后回收了2.9×10⁶ CFU ml⁻¹（回收率74%）。在80℃热处理10分钟后的单倍浓缩梨汁中的芽孢，在50℃下于pH 5.5的OSA平板上涂布培养5天后回收率更高（97%）。
结论
50℃下的PDA（pH 3.7）和OSA（pH 5.5）培养3 - 5天，从灭菌果汁和浓缩物中回收的 Alicyclobacillus 属细菌的营养细胞和芽孢数量最多。
研究的意义和影响
展示了从接种的果汁和浓缩物中回收 Alicyclobacillus 菌种最合适的合成培养基。
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