Rudi Knut, Skulberg Olav M, Jakobsen Kjetill S
Matforsk Norwegian Food Research Institute, As, Norway.
Prep Biochem Biotechnol. 2005;35(4):301-12. doi: 10.1080/10826060500218131.
The Planktothrix population in Lake Steinsfjord has attracted particular attention, due to the potential development of toxic blooms. This population is special in the sense that mass developments of Planktothrix occur in the metalimnion. We investigated the distribution of Planktothrix, as well as other cyanobacteria, through the water-column during a Planktothrix mass development at 10-16 m depth. The analyses were done by chlorophyll measurements, microscopy, and by a recently developed 16S rDNA array-based method. These analyses showed that Planktothrix dominated the cyanobacterial community at 11 m, while cyanobacteria belonging to the order Nostocales were predominant at 4 m. The combination of analytical methods presented in this work provides a powerful tool to analyze cyanobacterial communities. We have developed a concept that enables both relative (16S rDNA array analyses) and absolute quantification (chlorophyll a measurements) of cyanobacteria through water-columns. Such approaches will be important in better understanding cyanobacterial microbiota and bloom dynamics.
由于有毒水华可能出现，施泰因斯峡湾湖中的席藻种群受到了特别关注。该种群的特殊之处在于，席藻在温跃层大量繁殖。在席藻于10 - 16米深度大量繁殖期间，我们研究了席藻以及其他蓝细菌在水柱中的分布情况。分析通过叶绿素测量、显微镜检查以及一种最近开发的基于16S rDNA阵列的方法进行。这些分析表明，席藻在11米深处主导了蓝细菌群落，而在4米深处，念珠藻目的蓝细菌占主导地位。本文介绍的分析方法组合为分析蓝细菌群落提供了一个强大的工具。我们已经开发出一种概念，能够通过水柱对蓝细菌进行相对（16S rDNA阵列分析）和绝对定量（叶绿素a测量）。此类方法对于更好地理解蓝细菌微生物群和水华动态将具有重要意义。
