Asch R H, Patrizio P, Silber S J
Department of Obstetrics and Gynecology, University of California, Irvine.
Fertil Steril. 1992 Jul;58(1):190-3. doi: 10.1016/s0015-0282(16)55159-0.
To determine the ultrastructural features of epididymal and vasa efferentia sperm in men with congenital absence of the vas deferens.
Prospective.
University of California Irvine Center for Reproductive Health.
Thirteen men with surgical diagnosis of congenital absence of the vas deferens undergoing epididymal and vasa efferentia aspiration for assisted reproductive technology procedures.
The morphological quality and the integrity of the spermatozoa aspirated from the extratesticular segment of the rete testis, the vasa efferentia, and the caput epididymis were always markedly superior to those of sperm aspirated from the corpus and cauda epididymis, where the vast majority, if not all, were degenerating or frankly necrotic. The aspirates obtained from the distal segments of the epididymis also contained large numbers of sperm-laden macrophages; these were instead absent or exceptional in the fluids aspirated from the pre-epididymal portions of the excurrent pathways and from the caput of the epididymis.
This study demonstrates that the ultrastructural morphology of spermatozoa obtained by aspiration from the rete testis, vasa efferentia, and caput epididymis of individuals with congenital absence of the vas deferens is indistinguishable from that of spermatozoa in the semen.
确定先天性输精管缺如男性附睾及输出小管精子的超微结构特征。
前瞻性研究。
加利福尼亚大学欧文分校生殖健康中心。
13名经手术诊断为先天性输精管缺如的男性，接受附睾及输出小管穿刺以进行辅助生殖技术操作。
从睾丸网的睾丸外段、输出小管及附睾头吸出的精子的形态质量和完整性始终明显优于从附睾体和附睾尾吸出的精子，在附睾体和附睾尾，绝大多数（即便不是全部）精子正在退化或明显坏死。从附睾远端吸出的样本中还含有大量载有精子的巨噬细胞；而在从输出管道附睾前部分及附睾头吸出的液体中，这些细胞要么不存在，要么数量极少。
本研究表明，先天性输精管缺如个体经穿刺从睾丸网、输出小管及附睾头获取的精子的超微结构形态与精液中的精子无法区分。
