MacLachlan Ian, Cullis Pieter
Protiva Biotherapeutics Incorporated, Burnaby, BC, Canada V5G 4Y1.
Adv Genet. 2005;53:157-88.
Many viral and non-viral gene transfer systems suffer from common pharmacological issues that limit their utility in a systemic context. By application of the liposomal drug delivery paradigm, many of the limitations of the first generation non-viral delivery systems can be overcome. Encapsulation in small, long-circulating particles called stabilized plasmid lipid particles (SPLP) results in enhanced accumulation at disease sites and selective protein expression. This work compares the detergent dialysis method of SPLP manufacture with an alternative method, spontaneous vesicle formation by ethanol dilution. The pharmacology of SPLP, as determined by monitoring lipid label and quantitative real time PCR, is also presented.
许多病毒和非病毒基因传递系统都存在一些常见的药理学问题，这些问题限制了它们在全身应用中的效用。通过应用脂质体药物递送模式，可以克服第一代非病毒递送系统的许多局限性。封装在称为稳定化质粒脂质颗粒（SPLP）的小的、长循环颗粒中，可增强在疾病部位的积累和选择性蛋白质表达。这项工作将SPLP制备的去污剂透析方法与另一种方法——乙醇稀释自发形成囊泡法进行了比较。还介绍了通过监测脂质标记和定量实时PCR确定的SPLP的药理学。
