里氏木霉低分子量内切葡聚糖酶的分离与性质
Isolation and properties of a low molecular mass endoglucanase from Trichoderma reesei.
作者信息
Sprey B, Uelker A
机构信息
Institut für Biotechnologie, Forschungszentrum Jülich GmbH, FRG.
出版信息
FEMS Microbiol Lett. 1992 May 1;71(3):253-7. doi: 10.1016/0378-1097(92)90718-4.
Optimal culture conditions for obtaining low molecular mass endoglucanase (EG) from culture fluids of Trichoderma reesei were determined. The purification of this unglycosylated EG in a two-step procedure is described. In contrast to most cellulases, this EG did not bind to ConA-affinity columns. The unglycosylated fraction of the culture fluid proteins was further purified by preparative isoelectric focusing. Conditions relevant to an enzyme assay for this EG were determined (pH optimum 5.8, temperature optimum 52 degrees C).
确定了从里氏木霉培养液中获得低分子量内切葡聚糖酶（EG）的最佳培养条件。描述了通过两步法对这种未糖基化的EG进行纯化的过程。与大多数纤维素酶不同，这种EG不与伴刀豆球蛋白A亲和柱结合。培养液蛋白质的未糖基化部分通过制备性等电聚焦进一步纯化。确定了与该EG酶活性测定相关的条件（最适pH 5.8，最适温度52℃）。
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