通过使用光敏剂的可见光照射对培养细胞进行图案化处理。

Patterning cultured cells by visible light illumination with photosensitizers.

作者信息

Waki Ryoji, Gamo Sumiko, Bessho Masahiko, Hara Masayuki

机构信息

Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Sakai, Osaka 599-8570, Japan.

出版信息

J Biosci Bioeng. 2005 Sep;100(3):331-4. doi: 10.1263/jbb.100.331.

DOI:10.1263/jbb.100.331

PMID:16243285

Abstract

We showed that PC12 cells and 3T3 cells cultured in dishes were killed by illumination with visible white light from a halogen lamp at 7 x 10(4) lx for 5 min in the presence of either 10 microM hematoporphyrin or 2 microM methylene blue as a photosensitizer. This simple technique, based on the photodynamic reaction via generation of reactive oxygen species can be applicable for patterning cultured cells.

摘要

我们发现，在含有10微摩尔血卟啉或2微摩尔亚甲蓝作为光敏剂的情况下，培养皿中培养的PC12细胞和3T3细胞在7×10⁴勒克斯的卤钨灯可见光照射5分钟后被杀死。这种基于通过产生活性氧的光动力反应的简单技术可应用于对培养细胞进行图案化处理。