Ye Chunjiang, Wu Suowei, Yang Qingkai, Ma Chaozhi, Yang Guangsheng, Wang Bin
Huanzhong Agriculture University, Oilseed Rape Research Institute, Wuhan, 430070, P.R. China.
DNA Seq. 2005 Oct;16(5):364-71. doi: 10.1080/10425170500187791.
Agriculture productivity is severely hampered by soil salinity, drought and other environmental stresses. Studies on stress-resistant plants (halophytes, xerophytes, accumulating plants for specific toxic ions) have illuminated some mechanisms of stress tolerance in plants at metabolic or molecular levels, which gave some clues on how to genetically engineer stress-tolerant crops. With the isolation of more stress-responsive genes, genetic engineering with modified expression of stress responsive genes may be an effective way to produce stress-tolerant crops. In the present report, two genes (PEAMT and BADH) encoding the corresponding key enzymes for choline and glycine betaine (an important osmoprotectant) biosynthesis in plants were isolated in oilseed rape, an important oil crop in the world. Effects of salt stress on their expression were studied with quantitative PCR and their potential use in the genetic engineering of oilseed rape was discussed.
土壤盐渍化、干旱和其他环境胁迫严重阻碍了农业生产力。对抗逆植物（盐生植物、旱生植物、特定有毒离子积累植物）的研究在代谢或分子水平上揭示了植物一些耐胁迫机制，这为如何通过基因工程培育耐胁迫作物提供了一些线索。随着更多胁迫响应基因的分离，通过修饰胁迫响应基因的表达进行基因工程可能是培育耐胁迫作物的有效途径。在本报告中，从世界重要油料作物油菜中分离出两个编码植物中胆碱和甘氨酸甜菜碱（一种重要的渗透保护剂）生物合成相应关键酶的基因（PEAMT和BADH）。利用定量PCR研究了盐胁迫对它们表达的影响，并讨论了它们在油菜基因工程中的潜在用途。
