2-脱氧链霉胺生物合成早期中间体2-脱氧-scyllo-肌醇的生化研究。II. 2-脱氧-scyllo-肌醇的定量分析。
Biochemical studies on 2-deoxy-scyllo-inosose, an early intermediate in the biosynthesis of 2-deoxystreptamine. II. Quantitative analysis of 2-deoxy-scyllo-inosose.
作者信息
Yamauchi N, Kakinuma K
机构信息
Department of Chemistry, Tokyo Institute of Technology, Japan.
出版信息
J Antibiot (Tokyo). 1992 May;45(5):767-73. doi: 10.7164/antibiotics.45.767.
PMID:1624378
Abstract
A practical quantitative analysis of 2-deoxy-scyllo-inosose by means of GC-MS selected-ion monitoring (SIM) was exploited in order to assay the enzymatic 2-deoxy-scyllo-inosose formation, the first stage of the 2-deoxystreptamine biosynthesis. Mass spectral fragmentations of 2-deoxy-scyllo-inosose tetra-O-trimethylsilyl (TMS) ether was also investigated by the use of [2,2-2H2]-2-deoxy-scyllo-inosose.
摘要
利用气相色谱-质谱选择离子监测（SIM）对2-脱氧异肌醇进行了实际定量分析，以测定2-脱氧链霉胺生物合成第一阶段中酶促形成2-脱氧异肌醇的情况。还通过使用[2,2-2H2]-2-脱氧异肌醇研究了2-脱氧异肌醇四-O-三甲基硅烷基（TMS）醚的质谱碎裂情况。
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