Lu Wei-Hong, Wang Xin, Xu Li, Dai Yi-Jun, Yuan Sheng
Jiangsu Provincial Key Laboratory for Bioresource Technology, College of Life Science, Nanjing Normal University, Nanjing 210097, China.
Wei Sheng Wu Xue Bao. 2005 Aug;45(4):551-5.
Some features of Pseudomonas putida NA-1 strain in cultivation and enzyme production were different from S. marcescens IFO 12648 and P. fluorescens TN5 stains which could transform nicotinic acid to 6-hydroxynicotinic acid reported by other scientists, such as optimal carbon and its optimal concentration, optimal inducer concentration, and optimal cultivation temperature. The ideal transformation condition was nicotinic acid 3%, temperature 35 degrees C and pH 7.0. Under an appropriate condition, in a 4 liter fermenter, production yield of 6-hydroxynicotinic acid by Pseudomonas putida NA-1 was 108.39 g/L.
恶臭假单胞菌NA-1菌株在培养和酶生产方面的一些特征与粘质沙雷氏菌IFO 12648和荧光假单胞菌TN5菌株不同，其他科学家报道这两种菌株可将烟酸转化为6-羟基烟酸，例如最佳碳源及其最佳浓度、最佳诱导剂浓度和最佳培养温度。理想的转化条件是烟酸3%、温度35℃和pH值7.0。在合适的条件下，在4升发酵罐中，恶臭假单胞菌NA-1生产6-羟基烟酸的产量为108.39 g/L。
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