Lu Wei-hong, Xu Li, Dai Yi-jun, Yuan Sheng
Jiangsu Key Laboratory for Bioresource technology, NNU Laboratory of Microbiology and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210097, China.
Wei Sheng Wu Xue Bao. 2005 Feb;45(1):6-9.
Strain screening was conducted on a large scale in order to develop a biotransforming process for 6-hydroxynicotinic acid production. A strain named as NA-1 was isolated from soil which could transform nicotinic acid into a new product showed by HPLC. The new product was identified as 6-hydroxynicotinic acid by MS and 1H-NMR. The morphology, physiological and biochemical characteristics of strain NA-1 were studied and showed that characteristics of strain NA-1 were essentially consistent with Pseudomonas putida. The analysis of 16S rDNA sequence from strain NA-1 suggested that strain NA-1 was clustered together with P. putida in phylogenetic tree and the sequence identity between stain NA-1 and Pseudomonas putida was 99%. So strain NA-1 was identified to P. putida.
为开发一种生产6-羟基烟酸的生物转化工艺，进行了大规模的菌株筛选。从土壤中分离出一株名为NA-1的菌株，通过高效液相色谱法（HPLC）显示其能将烟酸转化为一种新产品。通过质谱（MS）和核磁共振氢谱（1H-NMR）鉴定该新产品为6-羟基烟酸。对菌株NA-1的形态、生理生化特性进行了研究，结果表明菌株NA-1的特性与恶臭假单胞菌基本一致。对菌株NA-1的16S rDNA序列分析表明，在系统发育树中菌株NA-1与恶臭假单胞菌聚在一起，菌株NA-1与恶臭假单胞菌之间的序列同一性为99%。因此，菌株NA-1被鉴定为恶臭假单胞菌。
