Tsuji Sumiko, Yoshii Kimihiko, Tonogai Yasuhide
National Institute of Health Sciences, Setagaya-ku, Tokyo 158-8501, Japan.
J Chromatogr A. 2006 Jan 6;1101(1-2):214-21. doi: 10.1016/j.chroma.2005.10.004. Epub 2005 Oct 24.
The existence of five of the six expected isomers in commercial Fast Green FCF (G3: Food Green No. 3, FD&C Green No. 3, CAS No. 2353-45-9, C.I. No. 42053), the main product of which is m,m-G3 and the sub-products of which are presumed to be m,p-G3, o,m-G3, p,p-G3, o,p-G3 and o,o-G3, was confirmed using LC/MS, and the levels of the isomers, m,m-G3, m,p-G3, p,p-G3, o,m-G3 and o,p-G3, were determined by analytical HPLC. The existence of seven subsidiary colors that were decomposed from G3 was also confirmed using LC/MS. The levels of the subsidiary colors in ethanol extracts from TLC were determined by HPLC and spectrophotometry, and these results were compared. It was clear that the values determined by TLC-spectrophotometry were higher than those by HPLC. It was recommended that the levels of subsidiary colors in G3 should be determined by HPLC.
商用亮绿FCF（G3：食用绿色3号、FD&C绿色3号、CAS编号2353-45-9、C.I.编号42053）中六种预期异构体中的五种已被确认存在，其主要产物为间，间-G3，推测副产物为间，对-G3、邻，间-G3、对，对-G3、邻，对-G3和邻，邻-G3，采用液相色谱/质谱联用（LC/MS）进行了确认，并且通过分析型高效液相色谱（HPLC）测定了间，间-G3、间，对-G3、对，对-G3、邻，间-G3和邻，对-G3这些异构体的含量。还采用LC/MS确认了由G3分解产生的七种副色的存在。通过HPLC和分光光度法测定了薄层色谱（TLC）乙醇提取物中副色的含量，并对这些结果进行了比较。很明显，TLC-分光光度法测定的值高于HPLC测定的值。建议采用HPLC测定G3中副色的含量。
