基于流体动力学方法的静脉注射肿瘤坏死因子α基因裸质粒DNA的抗肿瘤作用

Anti-tumor effect of intravenous TNFalpha gene delivery naked plasmid DNA using a hydrodynamics-based procedure.

作者信息

Kitajima Masayuki, Tsuyama Youichi, Miyano-Kurosaki Naoko, Takaku Hiroshi

机构信息

Department of Life and Environmental Science, Faculty of Engineering, Chiba Institute of Technology, Chiba, Japan.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):647-50. doi: 10.1081/ncn-200060136.

DOI:10.1081/ncn-200060136

PMID:16248005

Abstract

High levels of foreign gene expression in mouse hepatocytes can be achieved by the rapid injection of a large volume of naked plasmid (pDNA) into animals via the tail vein, the so-called hydrodynamics-based procedure. In this study, we evaluated the efficacy of hydrodynamics-based tumor necrosis factor alpha (TNFalpha) transfer for tumor treatment, in which the naked pDNA encoding TNFalpha was administered into the tail vein following an intravenous injection of B16 melanoma cells. The mice treated with TNFalpha-expressing pDNA displayed a profound reduction in lung metastasis. These results suggest that the hydrodynamics-based transfer of naked pDNA is a convenient and efficient method of TNFalpha gene therapy against metastatic tumors.

摘要

通过经尾静脉向动物快速注射大量裸质粒（pDNA），即所谓的基于流体动力学的方法，可以在小鼠肝细胞中实现高水平的外源基因表达。在本研究中，我们评估了基于流体动力学的肿瘤坏死因子α（TNFα）转移用于肿瘤治疗的疗效，其中在静脉注射B16黑色素瘤细胞后，将编码TNFα的裸pDNA经尾静脉给药。用表达TNFα的pDNA治疗的小鼠肺转移显著减少。这些结果表明，基于流体动力学的裸pDNA转移是一种针对转移性肿瘤的便捷且有效的TNFα基因治疗方法。