通过刚性或柔性连接子连接到DNA的苝：双链稳定性和荧光共振能量转移
Perylene attached to DNA through stiff or flexible linker: duplex stability and FRET.
作者信息
Skorobogatyj Mikhail V, Pchelintseva Anna A, Ustinov Alexei V, Korshun Vladimir A, Malakhov Andrei D
机构信息
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):931-4. doi: 10.1081/ncn-200059283.
PMID:16248065
Abstract
Two fluorescent nucleosides, 5-(perylen-3-ylethynyl)-2'-deoxyuridine and 5-[(perylen-3-yl)methoxypropyn-1-yl]-2'-deoxyuridine, were incorporated into synthetic oligodeoxyribonucleotides and spectral properties of the conjugates and their duplexes were studied.
摘要
将两种荧光核苷，即5-（苝-3-基乙炔基）-2'-脱氧尿苷和5- [（苝-3-基）甲氧基丙炔-1-基]-2'-脱氧尿苷，掺入合成的寡脱氧核糖核苷酸中，并研究了缀合物及其双链体的光谱性质。
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