T7 RNA聚合酶将核糖核苷5'-(α-P-硼烷)三磷酸掺入20聚体RNA中。

Incorporation of ribonucleoside 5'-(alpha-P-borano)triphosphates into a 20-mer RNA by T7 RNA polymerase.

作者信息

Wan Jing, Shaw Barbara Ramsay

机构信息

Department of Chemistry, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina, USA.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):943-6. doi: 10.1081/ncn-200059303.

DOI:10.1081/ncn-200059303

PMID:16248068

Abstract

The enzymatic synthesis of short boranophosphate RNA was studied by comparing the yield and pattern of abortive products of in vitro transcription at steady-state conditions with that of normal RNA. Boranophosphate short RNA can be readily synthesized by T7 RNA polymerase.

摘要

通过比较稳态条件下体外转录的流产产物与正常RNA的产量和模式，研究了短硼磷酸RNA的酶促合成。硼磷酸短RNA可以很容易地由T7 RNA聚合酶合成。