Duffy F J, Seiler J G, Hergrueter C A, Kandel J, Gelberman R H
Division of Plastic Surgery, Brigham and Women's Hospital, Boston.
J Hand Surg Br. 1992 Jun;17(3):275-7. doi: 10.1016/0266-7681(92)90114-h.
Recent studies have demonstrated an intrinsic neovascular response in intrasynovial healing tendons, introducing the possibility of mitogenic and/or angiogenic capability of intrasynovial tendon. To explore this hypothesis, healing canine flexor tendons were treated with early passive mobilization and the repair sites analysed at three, ten and 17 days. Specimens were mechanically digested and subjected to a standard BALB/c 3T3 mitogenic assay, which measures the capacity of tissue extracts to induce DNA synthesis and cell division in fibroblasts. Results revealed that both control and repaired flexor tendons possessed mitogenic activity, with the greatest activity observed in control specimens. Decreasing activity was noted as the time between repair and analysis increased. These data provide increasing evidence for the flexor tendon's active role in the healing process, and support the concept that mitogenic or growth-promoting factors are associated with flexor tendons and may be released following injury, during the early stages of healing.
近期研究已证实在滑膜内愈合的肌腱中存在内在的新生血管反应，这表明滑膜内肌腱具有促有丝分裂和/或血管生成的能力。为探究这一假说，对愈合中的犬屈肌腱进行早期被动活动，并在第3天、第10天和第17天对修复部位进行分析。将标本进行机械消化，并进行标准的BALB/c 3T3促有丝分裂试验，该试验可测量组织提取物诱导成纤维细胞中DNA合成和细胞分裂的能力。结果显示，对照和修复后的屈肌腱均具有促有丝分裂活性，对照标本中的活性最高。随着修复与分析之间时间的增加，活性降低。这些数据为屈肌腱在愈合过程中的积极作用提供了越来越多的证据，并支持这样的概念，即促有丝分裂或生长促进因子与屈肌腱相关，且可能在损伤后愈合早期释放。
