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通过选择离子流管质谱法(SIFT-MS)检测血培养培养基中细菌生长产生的挥发性代谢产物。

Detection of volatile metabolites produced by bacterial growth in blood culture media by selected ion flow tube mass spectrometry (SIFT-MS).

作者信息

Allardyce Randall A, Langford Vaughan S, Hill Alex L, Murdoch David R

机构信息

Department of Surgery, Christchurch School of Medicine and Health Sciences, Christchurch, New Zealand.

出版信息

J Microbiol Methods. 2006 May;65(2):361-5. doi: 10.1016/j.mimet.2005.09.003. Epub 2005 Oct 24.

Abstract

To achieve faster bacteremia diagnosis, selected ion flow tube mass spectrometry (SIFT-MS) measured metabolic gases in the headspaces of BacT/ALERT blood culture bottles. Pseudomonas aeruginosa, Streptococcus pneumoniae, Escherichia coli, Staphylococcus aureus and Neisseria meningitidis growth and trace gas patterns were detected from 10 colony forming units after 6 h.

摘要

为实现更快的菌血症诊断,采用选择离子流管质谱法(SIFT-MS)测量BacT/ALERT血培养瓶顶部空间中的代谢气体。6小时后,从10个菌落形成单位中检测到铜绿假单胞菌、肺炎链球菌、大肠杆菌、金黄色葡萄球菌和脑膜炎奈瑟菌的生长及痕量气体模式。

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