Cermák P, Sedláková P, Mejstríková L, Mazurová J
Institut of Clinical Microbiology Faculty of Medicine in Hradec Králové, Czech Republic.
Klin Mikrobiol Infekc Lek. 2006 Aug;12(4):156-60.
The article compares the ability of detection and detection times (TTD) of aerobic bacteria in the BacT/Alert FA, BacT/Alert SA, Bactec Plus Aerobic/F and Bactec Standard Aerobic/F bottles. Compared bottles were inoculated at the same time with the identical suspension of bacterial strain. All bottles detected bacterial pathogens even in the case of very low number of inoculated bacteria. The differences of TTD were detected between the bottles of compared hemocultivation systems. Bactec Plus Aerobic/F system is faster in the detection of the members of family Enterobacteriaceae at 1-3 hours, BacT/Aert FA system is faster in the detection of coagulase-negative staphylococci. Most difference was detected at Pseudomanas stutzeri, where the detection in BacT/Alert FA bottles was at 15 hours faster in opposite of Bactec Plus Aerobic/F bottles. In the bottles without sorbent Staphylococcus aureus and Streptococcus sanqui were detected faster in the system Bactec Standard Aerobic/F. The bottles BacT/Alert SA detected faster skin corynebacteria. Detected differences have not a practical importance for the blood stream infection diagnostics.
本文比较了BacT/Alert FA瓶、BacT/Alert SA瓶、Bactec Plus需氧/F瓶和Bactec标准需氧/F瓶中需氧菌的检测能力和检测时间(TTD)。将比较的瓶子同时接种相同的细菌菌株悬液。即使接种的细菌数量非常少,所有瓶子都能检测到细菌病原体。在比较的血培养系统的瓶子之间检测到了TTD的差异。Bactec Plus需氧/F系统在1 - 3小时内检测肠杆菌科成员更快,BacT/Aert FA系统在检测凝固酶阴性葡萄球菌方面更快。在斯氏假单胞菌中检测到的差异最大,在BacT/Alert FA瓶中的检测比Bactec Plus需氧/F瓶快15小时。在没有吸附剂的瓶子中,金黄色葡萄球菌和血链球菌在Bactec标准需氧/F系统中检测得更快。BacT/Alert SA瓶检测皮肤棒状杆菌更快。检测到的差异对血流感染诊断没有实际意义。