Lohmann C M, Riddiford L M
Department of Zoology, University of Washington, Seattle 98195.
J Exp Zool. 1992 Jul 1;262(4):374-82. doi: 10.1002/jez.1402620403.
In the tobacco hornworm, Manduca sexta, heat shock normally elicits synthesis of the classic heat-shock proteins. A 1-hr heat shock of 42 degrees C and above can also increase the relative synthesis of certain 12-18 kD proteins in the epidermis. These 12-18 kD proteins were identified as cuticular proteins for several reasons. Like cuticular proteins, they appear only in the epidermis. They can be precipitated from epidermal homogenates with an antiserum to larval cuticle. The same conditions that increased labeled 12-18 kD proteins in the epidermis also increased labeled 12-18 kD proteins in the cuticle. Some of the epidermal increase may result from a partial inhibition of secretion to the cuticle during 46 degrees C heat shock, causing abnormal accumulation in the epidermis. However, slight increases also occur at lower temperatures, which do not inhibit secretion detectably. Preliminary results also indicate that total quantities of at least one cuticular protein mRNA may increase during heat shock, either because of increased transcription or increased mRNA stability.
在烟草天蛾（Manduca sexta）中，热休克通常会引发经典热休克蛋白的合成。42摄氏度及以上的1小时热休克也会增加表皮中某些12 - 18千道尔顿蛋白质的相对合成量。这些12 - 18千道尔顿的蛋白质被鉴定为表皮蛋白，原因如下。与表皮蛋白一样，它们仅出现在表皮中。它们可以用针对幼虫表皮的抗血清从表皮匀浆中沉淀出来。在表皮中增加标记的12 - 18千道尔顿蛋白质的相同条件，也会增加表皮中标记的12 - 18千道尔顿蛋白质。表皮中的一些增加可能是由于在46摄氏度热休克期间对表皮分泌的部分抑制，导致在表皮中异常积累。然而，在较低温度下也会有轻微增加，而这些温度并不会明显抑制分泌。初步结果还表明，在热休克期间，至少一种表皮蛋白mRNA的总量可能会增加，这要么是由于转录增加，要么是由于mRNA稳定性增加。
