内消旋-α,α'-二氨基己二酸和内消旋-α,α'-二氨基辛二酸的双核2,2'-联吡啶-铂（II）和-钯（II）配合物的合成、光谱、细胞毒性及DNA结合研究

Synthesis, spectroscopic, cytotoxic, and DNA binding studies of binuclear 2,2'-bipyridine-platinum(II) and -palladium(II) complexes of meso-alpha,alpha'-diaminoadipic and meso-alpha,alpha'-diaminosuberic acids.

作者信息

Mansuri-Torshizi H, Srivastava T S, Parekh H K, Chitnis M P

机构信息

Department of Chemistry, Indian Institute of Technology, Powai, Bombay, India.

出版信息

J Inorg Biochem. 1992 Feb 1;45(2):135-48. doi: 10.1016/0162-0134(92)80008-j.

DOI:10.1016/0162-0134(92)80008-j

PMID:1624936

Abstract

Four new binuclear complexes of formula [M2(bipy)2(BAA)]Cl2 (where M is Pt(II) or Pd(II), bipy is 2,2'-bipyridine, and BAA is a dianion of meso-alpha-alpha'-diaminoadipic acid (DAA) or meso-alpha,alpha'-diaminosuberic acid (DSA) have been synthesized. These complexes have been characterized by chemical analysis and ultraviolet-visible, infrared, and 1H NMR spectroscopy. The mode of binding of ligands in these complexes has been ascertained by infrared and detailed 1H NMR spectroscopy. These complexes are 1:2 electrolyte in conductivity water. They have also been tested against P388 lymphocytic leukemia cells and their target is DNA molecules. [Pt2(bipy)2(DSA)]Cl2, [Pd2(bipy)2(DSA)Cl2, and [Pd2(bipy)2(DAA)]Cl2 show I.D.50 values comparable or lower than cis-diamminedichloroplatinum(II) and [Pt(bipy)(Ala)]Cl. In addition, binding studies of [Pt2(bipy)2(DSA)]Cl2 and [Pd2(bipy)2(DAA)]Cl2 to calf thymus DNA have been carried out and the mode of binding seems to be hydrogen bonding, as suggested earlier for analogous mononuclear amino acid-DNA complexes.

摘要

已合成了四种通式为[M2(bipy)2(BAA)]Cl2的双核配合物（其中M为Pt(II)或Pd(II)，bipy为2,2'-联吡啶，BAA为内消旋-α,α'-二氨基己二酸（DAA）或内消旋-α,α'-二氨基辛二酸（DSA）的二价阴离子）。这些配合物已通过化学分析以及紫外可见光谱、红外光谱和1H NMR光谱进行了表征。通过红外光谱和详细的1H NMR光谱确定了这些配合物中配体的结合模式。这些配合物在导电水中是1:2电解质。它们还针对P388淋巴细胞白血病细胞进行了测试，其作用靶点是DNA分子。[Pt2(bipy)2(DSA)]Cl2、[Pd2(bipy)2(DSA)Cl2和[Pd2(bipy)2(DAA)]Cl2的半数抑制浓度（ID50）值与顺二氯二氨合铂(II)和[Pt(bipy)(Ala)]Cl相当或更低。此外，还进行了[Pt2(bipy)2(DSA)]Cl2和[Pd2(bipy)2(DAA)]Cl2与小牛胸腺DNA的结合研究，结合模式似乎是氢键作用，正如之前对类似单核氨基酸-DNA配合物所提出的那样。