Berger M L, Veitl M, Malessa S, Sluga E, Hornykiewicz O
Institute of Biochemical Pharmacology, University of Vienna, Austria.
J Neurol Sci. 1992 Mar;108(1):114-7. doi: 10.1016/0022-510x(92)90196-r.
We analyzed binding sites for quinuclidinyl benzilate (QNB) and hemicholinium-3 (HC-3) by quantitative slice autoradiography and the activities of choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) in spinal cord of 5-7 patients with amyotrophic lateral sclerosis (ALS). In the ventral horn, QNB binding sites were markedly reduced (38% of controls; P less than 0.001), whereas HC-3 binding sites were only moderately affected (76%, P less than 0.01). Losses in cholinergic marker enzymes were inconsistent. The loss of muscarinic binding sites in the ventral horn was the most reliable cholinergic disease marker in ALS.
我们通过定量切片放射自显影分析了5-7例肌萎缩侧索硬化症（ALS）患者脊髓中苯甲托品（QNB）和半胱氨酸-3（HC-3）的结合位点，以及胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AChE）的活性。在腹角，QNB结合位点明显减少（为对照组的38%；P<0.001），而HC-3结合位点仅受到中度影响（76%，P<0.01）。胆碱能标记酶的损失并不一致。腹角中毒蕈碱结合位点的丧失是ALS中最可靠的胆碱能疾病标志物。
