Konomi Kenji, Zhu Cheng, Harris Deshea, Joyce Nancy C
Schepens Eye Research Institute and Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.
Invest Ophthalmol Vis Sci. 2005 Nov;46(11):4086-91. doi: 10.1167/iovs.05-0245.
To compare the relative proliferative capacity between human corneal endothelial cells (HCECs) cultured from the central and peripheral areas of the cornea.
Human corneas were divided into two groups based on donor age (younger group, < or =30 years of age; older group, > or =50 years of age). Corneas were trephined, and Descemet's membrane with HCECs was stripped from the central (0-6.75 mm) and peripheral (6.75-9.5 mm) areas. HCECs were then isolated from Descemet's membrane and cultivated. An equal number of passage-1 endothelial cells from each area were seeded, and the number of cells was determined at various times after seeding. Doubling times of cells from each area were compared. The antibody against minichromosome maintenance-2 (MCM2) protein was tested for replication competence.
Morphologically, HCECs from the central area were similar to cells from the peripheral area. The doubling time (in hours) of HCECs from the central area was 35.20 in the younger group (n = 4) and 54.54 in the older group (n = 4) and from the peripheral area, 29.37 in the younger group and 46.23 in the older group. There was no significant difference (younger: P = 0.515; older: P = 0.222) between the central and peripheral area in each age group. MCM2-positive cells were consistently observed in cultures from the central, as well as peripheral, area. There was no significant difference (younger: P = 0.929; older: P = 0.613) in the percentage of MCM2-positive cells between these two areas in either age group. Even though there was no significant difference, there was a tendency toward increased doubling time and decreased percentage of MCM2 in the central area of the older group.
These results indicate that corneal endothelial cells from both the central and peripheral areas retain potential proliferative capacity.
比较从角膜中央和周边区域培养的人角膜内皮细胞（HCECs）的相对增殖能力。
根据供体年龄将人角膜分为两组（年轻组，年龄≤30岁；老年组，年龄≥50岁）。将角膜环钻取材，从中央（0 - 6.75 mm）和周边（6.75 - 9.5 mm）区域剥离带有HCECs的Descemet膜。然后从Descemet膜中分离出HCECs并进行培养。接种来自每个区域的等量第1代内皮细胞，并在接种后的不同时间测定细胞数量。比较每个区域细胞的倍增时间。检测抗微小染色体维持蛋白2（MCM2）的抗体以评估复制能力。
形态学上，中央区域的HCECs与周边区域的细胞相似。年轻组（n = 4）中央区域HCECs的倍增时间（小时）为35.20，老年组（n = 4）为54.54；周边区域年轻组为29.37，老年组为46.23。每个年龄组的中央和周边区域之间无显著差异（年轻组：P = 0.515；老年组：P = 0.222）。在中央和周边区域的培养物中均持续观察到MCM2阳性细胞。两个年龄组中这两个区域之间MCM2阳性细胞的百分比无显著差异（年轻组：P = 0.929；老年组：P = 0.613）。尽管无显著差异，但老年组中央区域存在倍增时间增加和MCM2百分比降低的趋势。
这些结果表明，角膜中央和周边区域的内皮细胞均保留潜在的增殖能力。
