Tomassone L, Pagani P, De Meneghi D
Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università degli Studi di Torino, Grugliasco, Torino, Italy.
Parassitologia. 2005 Jun;47(2):247-51.
A reverse line blot hybridisation (RLB) assay was applied to screen Amblyomma variegatum adult ticks (n = 504) collected from N'Dama cattle in the Republic of Guinea. In a PCR, the V1 hypervariable region of the 16S ribosomal RNA (rRNA) gene was amplified with a set of primers unique for species of the genera Anaplasma and Ehrlichia, and the V4 hypervariable region of the 18S rRNA gene was amplified with primers specific for members of the genera Theileria and Babesia. Amplified PCR products from A. variegatum ticks were hybridised onto a membrane, to which oligonucleotide probes species-specific for Ehrlichia/Anaplasma and Theileria/Babesia parasites were covalently linked. No pathogens belonging to Ehrlichia/Anaplasma species were found, while 10 DNA samples resulted positive for Babesia caballi and 5 samples for Theileria velifera. This is the first report of B. caballi in A. variegatum ticks. One of the B. caballi positive samples was sequenced. This new strain (BcabGuinea) showed a 97% similarity to the Z15104 B. caballi GenBank sequence.
应用反向线杂交（RLB）分析法对从几内亚共和国的恩达马牛身上采集的504只成年变异革蜱进行检测。在聚合酶链反应（PCR）中，使用一组针对无形体属和埃立克体属物种的独特引物扩增16S核糖体RNA（rRNA）基因的V1高变区，并用针对泰勒虫属和巴贝斯虫属成员的特异性引物扩增18S rRNA基因的V4高变区。将变异革蜱的PCR扩增产物与一张膜杂交，该膜上共价连接了针对埃立克体/无形体和泰勒虫/巴贝斯虫寄生虫的物种特异性寡核苷酸探针。未发现属于埃立克体/无形体物种的病原体，而10份DNA样本检测出马巴贝斯虫呈阳性，5份样本检测出韦氏泰勒虫呈阳性。这是变异革蜱中首次报告马巴贝斯虫。对其中一份马巴贝斯虫阳性样本进行了测序。这个新菌株（BcabGuinea）与Z15104马巴贝斯虫基因库序列显示出97%的相似性。
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