山桦树叶伤口诱导的氧化反应。
Wound-induced oxidative responses in mountain birch leaves.
作者信息
Ruuhola Teija, Yang Shiyong
机构信息
Section of Ecology, Department of Biology, University of Turku, FIN-20014 Turku, Finland.
出版信息
Ann Bot. 2006 Jan;97(1):29-37. doi: 10.1093/aob/mcj005. Epub 2005 Oct 27.
AIMS
The aim of the study was to examine oxidative responses in subarctic mountain birch, Betula pubescens subsp. czerepanovii, induced by herbivory and manual wounding.
METHODS
Herbivory-induced changes in polyphenoloxidase, peroxidase and catalase activities in birch leaves were determined. A cytochemical dye, 3,3-diaminobenzidine, was used for the in situ and in vivo detection of H2O2 accumulation as a response to herbivory and wounding. To localize peroxidase activity in leaves, 10 mm H2O2 was applied to the dye reagent.
KEY RESULTS
Feeding by autumnal moth, Epirrita autumnata, larvae caused an induction in polyphenoloxidase and peroxidase activities within 24 h, and a concomitant decrease in the activity of antioxidative catalases in wounded leaves. Wounding also induced H2O2 accumulation, which may have both direct and indirect defensive properties against herbivores. Wound sites and guard cells showed a high level of peroxidase activity, which may efficiently restrict invasion by micro-organisms.
CONCLUSION
Birch oxidases together with their substrates may form an important front line in defence against herbivores and pathogens.
目的
本研究旨在检测亚北极山地桦（Betula pubescens subsp. czerepanovii）受食草动物取食和人工损伤诱导后的氧化反应。
方法
测定了桦树叶中多酚氧化酶、过氧化物酶和过氧化氢酶活性因食草动物取食而发生的变化。使用细胞化学染料3,3 - 二氨基联苯胺对因食草动物取食和损伤而积累的过氧化氢进行原位和体内检测。为了定位叶片中的过氧化物酶活性，将10 mM过氧化氢应用于染料试剂。
关键结果
秋尺蠖（Epirrita autumnata）幼虫取食在24小时内导致多酚氧化酶和过氧化物酶活性诱导增加，同时受伤叶片中抗氧化过氧化氢酶活性降低。损伤还诱导了过氧化氢的积累，其可能对食草动物具有直接和间接的防御特性。伤口部位和保卫细胞显示出高水平的过氧化物酶活性，这可能有效地限制微生物的入侵。
结论
桦树氧化酶及其底物可能在抵御食草动物和病原体方面形成重要的防线。
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