Ohno T, Kimura Y, Sakurada K, Sugimura K, Fujiyoshi T, Saheki T, Sonoda S, Azuma I
Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
Leuk Res. 1992;16(5):475-83. doi: 10.1016/0145-2126(92)90173-5.
Argininosuccinate synthetase (ASS) activity is hardly detected in human lymphocytes. In this study, we examined the ASS gene expression of various leukemia cells by a polymerase-chain-reaction method. We demonstrate here that (a) acute lymphocytic and acute myelocytic leukemia cells exhibit the highly elevated expression of the ASS gene and (b) chronic myelocytic leukemia (CML) in blastic crisis also exhibits the increase of ASS gene expression while CML in chronic phase, chronic lymphocytic leukemia and adult T leukemia cells show the similar level to that of normal lymphocytes. These results suggest that the ASS gene expression is of value as a diagnostic marker of acute type leukemia, particularly for blastic crisis of CML.
精氨琥珀酸合成酶（ASS）活性在人类淋巴细胞中几乎检测不到。在本研究中，我们通过聚合酶链反应方法检测了各种白血病细胞的ASS基因表达。我们在此证明：（a）急性淋巴细胞白血病细胞和急性髓细胞白血病细胞表现出ASS基因的高度表达升高；（b）处于原始细胞危象的慢性髓细胞白血病（CML）也表现出ASS基因表达增加，而慢性期的CML、慢性淋巴细胞白血病和成人T白血病细胞显示出与正常淋巴细胞相似的水平。这些结果表明，ASS基因表达作为急性型白血病尤其是CML原始细胞危象的诊断标志物具有价值。
