Tokumura A, Yotsumoto T, Hoshikawa T, Tanaka T, Tsukatani H
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Life Sci. 1992;51(4):303-8. doi: 10.1016/0024-3205(92)90089-8.
Age-related decrease of the platelet-activating factor (PAF) content in rat brain was shown by a convenient method consisting of solid extraction of lipids with a Sep-Pak C-18 cartridge, lipid separation by HPLC and bioassay on rabbit platelets. This method was sufficiently sensitive to allow measurement of PAF in a single brain, and the recovery of PAF was quite high throughout the procedure.
