[The relationship between TNF-alpha and the injury of renal tubular cells caused by anoxia/reoxygenation].
作者信息
Dong Wen-bin, Du Yi-ting, Chen Yue, Hang Yong-lun, Chen Feng, Deng Cun-liang
机构信息
Department of Newborn Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005 Nov;21(6):690-2.
AIM
To investigate the relationship between TNF-alpha and renal tubular cell injury caused by anoxia/reoxygenation.
METHODS
Human renal proximal tubular cell line HK-2 was used as model. Anoxia/reoxygenation were produced by covering/de-covering the cell culture with liquid paraffin wax. The level of TNF-alpha and the activity of lactate dehydrogenase(LDH) in the culture medium was determined by radioimmunoassay(RIA) and biochemical methods, respectively. Trypan blue exclusion was used to measure cell viability.
RESULTS
Anoxia/reoxygenation could increase TNF-alpha level and LDH activity, but decrease viability of HK-2 cells. TNF-alpha level was positively correlated with LDH activity (r=0.89, P<0.05) and negatively with the cell viability (r=-0.91, P<0.05).
CONCLUSION
TNF-alpha induced by anoxia/reoxygenation may participate in the process of renal tubular cell injury.
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