[Replication of a model of injury to human renal proximal tubular cells induced by hypoxia/reoxygenation].

OBJECTIVE

To replicate a new model of injury to human renal proximal tubular cells (HK-2) induced by hypoxia/reoxygenation.

METHODS

Human renal proximal tubular cell line HK-2 cell was used as the target cell. Tubular cells were divided into six groups: 4 hours of hypoxia, 12 hours of hypoxia, 24 hours of hypoxia, and 24 hours of hypoxia followed by reoxygenation 4, 12 or 24 hours later groups. Each group was accompanied by a control group. Hypoxic culture conditions were produced by covering the culture with liquid paraffin. Trypan blue exclusion was used for cell count and cell viability. The activity of lactate dehydrogenase (LDH) in the culture medium was determined by biochemical method.

RESULTS

After being challenged by hypoxia followed by reoxygenation, trypan blue exclusion rate was greater, cell count and cell viability were lower, and the activity of LDH was increased. It indicated that the destruction of integrity of cellular membrane was induced by ischemia/reperfusion injury, and the tubular cells may be injured irreversibly.

CONCLUSION

A simple model of hypoxic injury of renal tubular cells is replicated by covering the culture cells with liquid paraffin.