Szołtys Maria, Słomczyńska Maria, Duda Małgorzata, Sakiewicz Anna, Otak Agnieszka
Laboratory of Endocrinology and Tissue Culture, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Kraków, Poland.
Acta Histochem. 2005;107(5):357-64. doi: 10.1016/j.acthis.2005.09.002. Epub 2005 Oct 25.
The immunohistochemical localisation of androgen receptor (AR) was investigated in a cohort of ovarian antral follicles developing, and subsequently undergoing atresia, in a hyperprolactinaemic milieu at the beginning of pregnancy in rats. Differentiation of the investigated follicles, observed during the first 5 days of pregnancy, was accompanied by a centripetal disappearance of androgen nuclear receptor in the granulosa layer, which did not include the cumulus oophorus complex and some antral granulosa cells. This pattern of decline resembled that typical of follicles maturing during the oestrous cycle but took longer to occur. The follicles did not ovulate and subsequently underwent atresia. The degeneration of some follicles was accompanied by a further loss of AR in the cumulus granulosa cells, but a strong positive AR immunoreaction persisted in the oocyte nucleoli. Some perinatal and early antral atretic follicles were found. In most cases their granulosa layers were AR-positive, although often only weakly. However, follicles with AR negative granulosa layers were also encountered. Nuclear immunolabelling for AR was positive in luteinized follicles. It can be concluded that follicular atresia involves changes in AR distribution which can be demonstrated as an abnormal depletion or persistence of AR.
在大鼠妊娠初期的高泌乳素环境中，对一组正在发育并随后发生闭锁的卵巢窦状卵泡进行雄激素受体（AR）的免疫组化定位研究。在妊娠的前5天观察到，所研究卵泡的分化伴随着颗粒层中雄激素核受体向心式消失，这并不包括卵丘复合体和一些窦状颗粒细胞。这种下降模式类似于发情周期中成熟卵泡的典型模式，但发生时间更长。这些卵泡没有排卵，随后发生闭锁。一些卵泡的退化伴随着卵丘颗粒细胞中AR的进一步丧失，但在卵母细胞核仁中仍存在强烈的AR免疫反应阳性。发现了一些围产期和早期窦状闭锁卵泡。在大多数情况下，它们的颗粒层AR呈阳性，尽管通常较弱。然而，也遇到了颗粒层AR阴性的卵泡。黄体化卵泡中AR的核免疫标记呈阳性。可以得出结论，卵泡闭锁涉及AR分布的变化，这可以表现为AR的异常耗竭或持续存在。
