p63在涎腺多形性腺瘤及多形性腺瘤癌变中的免疫组化表达
Immunohistochemical expression of p63 in pleomorphic adenomas and carcinomas ex-pleomorphic adenomas of salivary glands.
作者信息
Genelhu Marisa Cristina Leite Santos, Gobbi Helenice, Soares Fernando Augusto, Campos Antônio Hugo José Froes de Marques, Ribeiro Carlos Alberto, Cassali Geovanni Dantas
机构信息
Laboratório de Patologia Comparada, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
出版信息
Oral Oncol. 2006 Feb;42(2):154-60. doi: 10.1016/j.oraloncology.2005.06.026. Epub 2005 Oct 26.
Alteration of the p63 protein expression has been associated with several human cancers including salivary gland tumors. We sought to assess the p63 expression in pleomorphic adenomas (PA) and carcinoma ex-pleomorphic adenomas (Ca ex-PA), since this protein has been related to myoepithelial phenotype and the biological behavior of cancer. Ten samples of PA and ten of Ca ex-PA were submitted to immunohistochemistry using a monoclonal anti-p63 antibody, clone 4A4. p63 was regularly expressed in myoepithelial cells' nuclei of the normal glandular parenchyma and in PA, but it was completely absent in five out of the ten samples of Ca ex-PA. (P < 0.05 by Fisher's exact test). Apparently, only those cases depicting some myoepithelial differentiation were positive. Our data suggest that loss of myoepithelial differentiation is important in the evolution of Ca ex-PA, and corroborate the hypothesis that p63 antigen may be a useful marker of myoepithelial cells in salivary glands neoplasms.
p63蛋白表达的改变与包括涎腺肿瘤在内的多种人类癌症相关。我们试图评估多形性腺瘤（PA）和多形性腺瘤恶变（Ca ex-PA）中的p63表达情况，因为这种蛋白与肌上皮表型及癌症的生物学行为有关。使用单克隆抗p63抗体（克隆号4A4）对10例PA样本和10例Ca ex-PA样本进行免疫组织化学检测。p63在正常腺实质的肌上皮细胞核以及PA中呈规律性表达，但在10例Ca ex-PA样本中有5例完全缺失（Fisher精确检验，P<0.05）。显然，只有那些表现出一定肌上皮分化的病例呈阳性。我们的数据表明，肌上皮分化的丧失在Ca ex-PA的演变过程中很重要，并证实了p63抗原可能是涎腺肿瘤中肌上皮细胞有用标志物的假说。
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