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土拉弗朗西斯菌活疫苗株的灰色变体缺乏脂多糖O抗原,在巨噬细胞中的存活能力降低,并且不能在小鼠中诱导保护性免疫。

Grey variants of the live vaccine strain of Francisella tularensis lack lipopolysaccharide O-antigen, show reduced ability to survive in macrophages and do not induce protective immunity in mice.

作者信息

Hartley Gill, Taylor Rosa, Prior Jo, Newstead Sarah, Hitchen Paul G, Morris Howard R, Dell Anne, Titball Richard W

机构信息

Defence Science and Technology Laboratory, Salisbury, Wiltshire, UK.

出版信息

Vaccine. 2006 Feb 13;24(7):989-96. doi: 10.1016/j.vaccine.2005.08.075. Epub 2005 Sep 6.

Abstract

Francisella tularensis live vaccine strain (LVS) produces two colony types when grown on solid media, often referred to as blue variants (BV) and grey variants (GV). Whereas blue variant bacteria possessed a lipopolysaccharide O-side chain, grey variant bacteria lacked O-side chains. Grey variant bacteria appeared in stationary phase bacterial cultures and could be identified using a novel FACS-based assay. Compared to blue variant bacteria, grey variants showed a reduced ability to infect and survive in macrophages. The immunisation of mice with blue variant bacteria, but not grey variant bacteria, induced protective immunity towards fully virulent F. tularensis.

摘要

土拉弗朗西斯菌活疫苗株(LVS)在固体培养基上生长时会产生两种菌落类型,通常称为蓝色变体(BV)和灰色变体(GV)。蓝色变体细菌具有脂多糖O侧链,而灰色变体细菌缺乏O侧链。灰色变体细菌出现在稳定期细菌培养物中,可通过一种基于流式细胞术的新型检测方法进行鉴定。与蓝色变体细菌相比,灰色变体在巨噬细胞中的感染和存活能力降低。用蓝色变体细菌而非灰色变体细菌免疫小鼠,可诱导对完全有毒力的土拉弗朗西斯菌的保护性免疫。

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