Wang Chun-Yu, Shi Jian-Dang, Zhu Yan, Zhang Ju
The Key Laboratory of Bioactive Materials, Ministry of Education, Institute for Molecular Biology, Nankai University, Tianjin 300071, China.
Yi Chuan. 2005 Sep;27(5):801-7.
In the post-genomic era, identifying and characterizing various DNA-protein interactions are a major challenge in the research of gene transcriptional regulation. Although many techniques can be used for this purpose, chromatin immunoprecipitation assay (ChIP), by contrast, is ideally suited for studying DNA-protein interactions in vivo. In recent years, standard ChIP assay has been modified to uncover some known factors' unknown target sequences, especially when combined with DNA microarray and molecular cloning strategies. These high-throughput ChIP assays are more and more used to reveal the distribution profile of trans-acting factor binding sites throughout the genome, which may yield many new insights into the DNA-protein interaction network. This article summarized the methods of ChIP assay, and highlighted recent progress in the application of this technique.
在后基因组时代，识别和表征各种DNA-蛋白质相互作用是基因转录调控研究中的一项重大挑战。尽管有许多技术可用于此目的，但相比之下，染色质免疫沉淀测定法（ChIP）非常适合在体内研究DNA-蛋白质相互作用。近年来，标准的ChIP测定法已被改进，以发现一些已知因子的未知靶序列，特别是当与DNA微阵列和分子克隆策略结合使用时。这些高通量ChIP测定法越来越多地用于揭示全基因组中转录因子结合位点的分布图谱，这可能会为DNA-蛋白质相互作用网络带来许多新的见解。本文总结了ChIP测定法的方法，并重点介绍了该技术应用的最新进展。
