Lindefors N, Boatell M L, Mahy N, Persson H
Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Neurosci Lett. 1992 Feb 3;135(2):262-4. doi: 10.1016/0304-3940(92)90451-c.
In efforts to test the cholinergic hypothesis for Alzheimer's disease and to create an animal model for this disease, ibotenic acid has been used to lesion cholinergic neurons in the basal forebrain. In this study we have used in situ hybridization with oligonucleotide probes specific for mRNAs encoding choline acetyltransferase and glutamic acid decarboxylase, respectively, to study the effects of such a lesion. Our results show that lesion paradigms normally used to induce neuronal degeneration in nucleus basalis by ibotenic acid not only lesion the cholinergic neurons within this nucleus, but in addition, a major fraction of gamma-aminobutyric acid (GABA) neurons in nucleus basalis, substantia innominata, globus pallidus and ventral pallidum.
为了验证阿尔茨海默病的胆碱能假说并建立该疾病的动物模型，鹅膏蕈氨酸已被用于损毁基底前脑的胆碱能神经元。在本研究中，我们分别使用与编码胆碱乙酰转移酶和谷氨酸脱羧酶的mRNA特异性寡核苷酸探针进行原位杂交，以研究这种损毁的影响。我们的结果表明，通常用于通过鹅膏蕈氨酸诱导基底核神经元变性的损毁模式不仅损毁了该核内的胆碱能神经元，而且还损毁了基底核、无名质、苍白球和腹侧苍白球中很大一部分γ-氨基丁酸（GABA）能神经元。
