Deveci Figen, Akbulut H Handan, Turgut Teyfik, Muz M Hamdi
Department of Chest Diseases, Firat Medical Center, Faculty of Medicine, Firat University, Elazig, Turkey.
Mediators Inflamm. 2005 Oct 24;2005(5):256-62. doi: 10.1155/MI.2005.256.
It has been reported that IFN-gamma, TNF-alpha, and IL-12 stimulate, and that IL-10, TGF-beta, and IL-4 suppress the protective immune response against tuberculosis. We aim to evaluate changes in the serum levels of pro and antiinflammatory cytokines in active pulmonary tuberculosis (APTB) and the possible effects of treatment on these changes. Serum IL-12p40, IL-4, IL-10, TNF-alpha, IFN-gamma, and TGF-beta1 levels were determined in 20 APTB cases (group 1) before and 2, 4, and 6 months after therapy. The same parameters were also determined in 9 inactive pulmonary tuberculosis (IPTB) cases (group 2) and 9 healthy controls (HC, group 3). Before treatment, the mean serum IFN-gamma, TNF-alpha, and IL-10 levels in group 1 were statistically higher than those in group 2 (P=.001, P=.024, P=.016, resp) or group 3 (P=.003, P=.002, P=.011, resp). The levels in group 1 decreased significantly after treatment (P=.001 for IFN-gamma, P=.004 for TNF-alpha, P=.000 for IL-10). The serum levels of IL-12p40 were significantly higher in group 1 than in group 3 (P=.012) and decreased insignificantly after treatment. There was no difference in serum IL-4 and TGF-beta1 levels among the groups (P>.05). Because the serum IL-12p40, IL-10, TNF-alpha, and IFN-gamma levels were high in APTB, we believe that these cytokines have important roles in the immune response to Mycobacterium tuberculosis (M tuberculosis). These parameters could be used in follow-up as indicators of the success of APTB therapy.
据报道，γ干扰素、肿瘤坏死因子-α和白细胞介素-12具有刺激作用，而白细胞介素-10、转化生长因子-β和白细胞介素-4则抑制针对结核病的保护性免疫反应。我们旨在评估活动性肺结核（APTB）患者血清中促炎和抗炎细胞因子水平的变化以及治疗对这些变化可能产生的影响。测定了20例APTB患者（第1组）治疗前以及治疗后2、4和6个月时血清白细胞介素-12p40、白细胞介素-4、白细胞介素-10、肿瘤坏死因子-α、γ干扰素和转化生长因子-β1的水平。还测定了9例非活动性肺结核（IPTB）患者（第2组）和9名健康对照者（HC，第3组）的相同参数。治疗前，第1组血清γ干扰素、肿瘤坏死因子-α和白细胞介素-10的平均水平在统计学上高于第2组（分别为P = 0.001、P = 0.024、P = 0.016）或第3组（分别为P = 0.003、P = 0.002、P = 0.011）。第1组治疗后这些水平显著下降（γ干扰素P = 0.001，肿瘤坏死因子-αP = 0.004，白细胞介素-10P = 0.000）。第1组血清白细胞介素-12p40水平显著高于第3组（P = 0.012），治疗后略有下降。各组血清白细胞介素-4和转化生长因子-β1水平无差异（P>0.05）。由于APTB患者血清白细胞介素-12p40、白细胞介素-10、肿瘤坏死因子-α和γ干扰素水平较高，我们认为这些细胞因子在针对结核分枝杆菌（M tuberculosis）的免疫反应中起重要作用。这些参数可作为APTB治疗成功与否的指标用于随访。
