有证据表明酵母纺锤体装配检查点除了后期促进复合物之外还有其他靶点。

Evidence that the yeast spindle assembly checkpoint has a target other than the anaphase promoting complex.

作者信息

Andrews Catherine A, Díaz-Martínez Laura A, Giménez-Abián Juan F, Guacci Vincent, Vas Amit C, Clarke Duncan J

机构信息

Department of Genetics, Cell Biology & Development, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA.

出版信息

Cell Cycle. 2005 Nov;4(11):1555-7. doi: 10.4161/cc.4.11.2144. Epub 2005 Nov 5.

DOI:10.4161/cc.4.11.2144

PMID:16258265

Abstract

The spindle assembly checkpoint monitors biorientation of chromosomes on the metaphase spindle and inhibits the Anaphase Promoting Complex (APC) specificity factor Cdc20. If APC-Cdc20 is the sole target of the spindle checkpoint, then cells lacking APC and its targets, B-type cyclin and securin, would lack spindle checkpoint function. We tested this hypothesis in yeast cells that are APC-null. Surprisingly, we find that such yeast cells are able to activate the spindle assembly checkpoint, delaying cell cycle progression in G2/M phase. These data suggest that the spindle checkpoint has a non-APC target that can restrain anaphase onset.

摘要

纺锤体组装检验点监测中期纺锤体上染色体的双定向，并抑制后期促进复合物（APC）特异性因子Cdc20。如果APC-Cdc20是纺锤体检验点的唯一靶点，那么缺乏APC及其靶点（B型细胞周期蛋白和分离酶）的细胞将缺乏纺锤体检验点功能。我们在APC缺失的酵母细胞中验证了这一假设。令人惊讶的是，我们发现这类酵母细胞能够激活纺锤体组装检验点，使细胞周期进程在G2/M期延迟。这些数据表明，纺锤体检验点有一个非APC靶点，可抑制后期起始。